ZIKV -Organoids

Published: 18 June 2024| Version 1 | DOI: 10.17632/8swwg9jp6n.1
Contributor:
Isabel Pagani

Description

Preliminary data of ZIKV infection in brain organoids. We perform immunostaining to characterise organoids grown in culture for 5 weeks. We identified neurons (MAP2) and astrocytes (GFAP). We tested the cytopathic effect of ZIKV in this 3D-system after infection with 1.000.000 infectious particles. We took pictures every 3 days post-infection by optic microscope and collected the supernatant for the retrotitration through plaque forming assay to check viral replication.

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Institutions

  • Ospedale San Raffaele

Categories

Virus, Neuroprotection, Organoid, Drug Repositioning

Funders

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