ZIKV -Organoids
Published: 18 June 2024| Version 1 | DOI: 10.17632/8swwg9jp6n.1
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Description
Preliminary data of ZIKV infection in brain organoids. We perform immunostaining to characterise organoids grown in culture for 5 weeks. We identified neurons (MAP2) and astrocytes (GFAP). We tested the cytopathic effect of ZIKV in this 3D-system after infection with 1.000.000 infectious particles. We took pictures every 3 days post-infection by optic microscope and collected the supernatant for the retrotitration through plaque forming assay to check viral replication.
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Institutions
Ospedale San Raffaele
Categories
Virus, Neuroprotection, Organoid, Drug Repositioning